Luminex 100 IS Developer Workbench Guide Version 2.3 Manuale Utente Pagina 62

  • Scaricare
  • Aggiungi ai miei manuali
  • Stampa
  • Pagina
    / 74
  • Indice
  • SEGNALIBRI
  • Valutato. / 5. Basato su recensioni clienti
Vedere la pagina 61
Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology
56 PN 89-00002-00-084 Rev. B
Oligonucleotide Coupling Protocol
Introduction Use these protocols as a general starting point for developing assays.
Optimize all assays for your reagents in your specific application.
Updates or additions to these protocols are posted on the Luminex
website at
http://luminexcorp.custhelp.com. At the main page, select
a subject or perform a search for the desired information.
You obtain the best results by starting with these guidelines and
modifying them for your specific needs.
Equipment •Vortex
Sonicator bath
Micropipetters: (1 µL - 1000 µL)
Microcentrifuge
Microfuge tubes (USA Scientific)
•Timer
Analytical balance
Materials xMAP carboxylated microspheres—LIMIT EXPOSURE TO
LIGHT!
Amino-substituted oligonucleotide (See Technical note 1)
Microcentrifuge tubes: 1.5 mL, polypropylene (see the following
technical notes)
Pipetter tips: 1 µL - 1000 µL
•Pierce EDC:
1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
•MES:
0.1 M 2- (N-morpholino) ethane sulfonic acid pH 4.5, filter,
sterilize, and store at 4°C.
Tween-20 (0.02% v/v)
•SDS:
Sodium Dodecyl Sulfate (0.1% w/v)
•dH
2
O
TE Buffer (10mM Tris, 1mM EDTA, pH 8.0)
Preparation 1. Allow all reagents to warm to room temperature.
2. Resuspend the amine-substituted oligonucleotide to 1.0 mM in
sterile, deionized water.
Vedere la pagina 61
1 2 ... 57 58 59 60 61 62 63 64 65 66 67 ... 73 74

Commenti su questo manuale

Nessun commento