Luminex 100 IS Version 2.3 Manuale Utente

Luminex 100™ IS User Manual Version 2.3 Lum/nex®

iv PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyMaximize/Minimize. . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 58 PN 89-00002-00-072 Rev. C Delete a Batch You can only delete unprocessed batches. Batche

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 59Figure 5-30. Luminex Multi-Batch Setup Dialog Box2. In the Luminex

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 60 PN 89-00002-00-072 Rev. C 3. Select the first batch to add and click Select. The batch a

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 61Figure 5-31. Processing Multiple PlatesAfter the first microtiter

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 62 PN 89-00002-00-072 Rev. C  To open an incomplete batch:1. On the File Menu, click Open

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 63Where x = accession ID number for the patient (patient identifier s

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 64 PN 89-00002-00-072 Rev. C system appends any remaining patient IDs to the end of the com

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 65Figure 5-33. Edit Patient List Dialog BoxAssign SampleDilution Fac

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 66 PN 89-00002-00-072 Rev. C Figure 5-34. Luminex Batch Setup for Dilution4. To change the

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 672. On the Acquisition Detail toolbar, click New Advanced Batch. The

PN 89-00002-00-072 Rev. C vxMAP Technology ContentsDefine Data Export Tab Information . . . . . . . . . . . . . . . .5-41Setting up the Favorites Li

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 68 PN 89-00002-00-072 Rev. C Figure 5-36. Options Dialog Box—Bead Set Tab7. Edit the Event

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 69 Figure 5-37. Plate Layout Tab9. Select wells. To select a single

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 70 PN 89-00002-00-072 Rev. C 11. Establish preliminary off-plate commands. The system perfo

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 71To establish insert off-plate commands, select the desired wells, t

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 72 PN 89-00002-00-072 Rev. C Create a New Lot  To create a new lot:1. On the Home tab, cli

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 73Figure 5-43. Update Lot Information Dialog Box4. Enter the expirat

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 74 PN 89-00002-00-072 Rev. C Figure 5-44. Update Lot Information Dialog Box3. Change or e

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 755. Respond to the Create New Standard Lot or Create New Control Lot

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 76 PN 89-00002-00-072 Rev. C Export a Lot froman ExistingTemplateUse this procedure to expo

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 77There are two main assay types: non-competitive (such as a “capture

vi PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyDatabase Management Procedures . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 78 PN 89-00002-00-072 Rev. C 3. Double-click anywhere within the graph to display the Custo

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 79 Figure 5-47. Exporting Dialog BoxMaximize: Click to maximize the

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 80 PN 89-00002-00-072 Rev. C Grid in front of data: Check this option to place the grid in

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 81Desk Background: this is the color that surrounds the bound-ing rec

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 82 PN 89-00002-00-072 Rev. C Figure 5-51. Customization Dialog Box—Style Tab To modify fe

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 83To ensure that calculated data is exported to the output.csv file,

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 84 PN 89-00002-00-072 Rev. C View Detailed TestAnalysis To view detailed test analysis:1.

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 85 1. Menu for multiple tests 2. Men

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 86 PN 89-00002-00-072 Rev. C analysis. Figure 5-55 shows standard and control. Figure 5-56

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 87Running Reports and AnalysesYou can output data by printing reports

PN 89-00002-00-072 Rev. C viixMAP Technology ContentsPressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 88 PN 89-00002-00-072 Rev. C Figure 5-57. Report Selection Dialog Box2. Select the type of

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 89Figure 5-59. Patient Selection Dialog Box4. Select the desired ent

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 90 PN 89-00002-00-072 Rev. C Print DataAnalysis ReportA printed batch report includes the f

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 91Figure 5-61. Data Interpretation Report (Print Preview)Database Ma

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 92 PN 89-00002-00-072 Rev. C  To back up the database:1. On the Tools menu, click Database

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 933. From the Restore Database From dialog box, select a database bac

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 94 PN 89-00002-00-072 Rev. C Drain the Analyzer When draining, you do not need to supply so

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 95 To drain the system:1. On the Maintenance tab, click Eject/Retrac

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 96 PN 89-00002-00-072 Rev. C Figure 5-62. Cleanup Utility Dialog Box To perform a disk c

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 97 To delete the batch folders:1. In the Cleanup Utility dialog box,

viii PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyRemove Luminex LMAT Software . . . . . . . . . . . . . . . . B-

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 98 PN 89-00002-00-072 Rev. C Perform a SoakCommandSoak the sample probe to prevent the shea

PN 89-00002-00-072 Rev. C 6 - 1Maintenance and CleaningTo ensure accurate test results, properly clean and maintain the Luminex 100 IS system. Read

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 2 PN 89-00002-00-072 Rev. C 5. Click Wash. In the confirmation dialog box, click on the dro

xMAP Technology Maintenance and CleaningPN 89-00002-00-072 Rev. C 6 - 3Refill the SheathFluid Container To refill the sheath fluid container:1. Rel

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 4 PN 89-00002-00-072 Rev. C Flush the System Run 3 backflushes, 3 drains, 2 alcohol flushes

xMAP Technology Maintenance and CleaningPN 89-00002-00-072 Rev. C 6 - 53. Open both doors of the analyzer and clean all accessible surfaces with det

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 6 PN 89-00002-00-072 Rev. C Figure 6-2 Grasping the Tubing4. Remove the filter with one ha

xMAP Technology Maintenance and CleaningPN 89-00002-00-072 Rev. C 6 - 7 Figure 6-3 Removing the Screen3. Replace the filter and replace the screen.

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 8 PN 89-00002-00-072 Rev. C Syringe Seal  To replace the syringe plunger seal:1. Disconnec

xMAP Technology Maintenance and CleaningPN 89-00002-00-072 Rev. C 6 - 911. Hand-tighten the screw on the syringe arm.12. Plug in the power cord and

PN 89-00002-00-072 Rev. C 1 - 1IntroductionAbout This Manual This manual provides you with information to understand and use the Luminex® 100™ IS sy

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 10 PN 89-00002-00-072 Rev. C AnnuallySheath Filter  To change the Luminex 100 analyzer she

xMAP Technology Maintenance and CleaningPN 89-00002-00-072 Rev. C 6 - 11As requiredFuses To replace the fuses in either the Luminex 100 analyzer or

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 12 PN 89-00002-00-072 Rev. C

Luminex 100 IS Maintenance Log Month(s): ________________________________________Year:_______________Maintenance LogProcedure

PN 89-00002-00-072 Rev. C 7 - 1 TroubleshootingTroubleshooting the Luminex 100 IS SystemTroubleshooting procedures help users isolate, identify, and

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 2 PN 89-00002-00-072 Rev. C Friday. Inquiries may also be sent by email to support@luminexco

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 3Pressurization Normal air and sheath pressure readings vary between 6-9 psi while the c

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 4 PN 89-00002-00-072 Rev. C Fluid Leaks Fluid leaks can result in poor pressurization and fa

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 5Sample Probe Problems with the sample probe can lead to fluid leaks and pressurization

Luminex 100 IS User Manual Version 2.3 xMAP Technology1 - 2 PN 89-00002-00-072 Rev. C Technical Support Luminex Technical Support representatives are

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 6 PN 89-00002-00-072 Rev. C Sample arm stuck in the down position.The sample probe height is

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 7Calibration and Control ProblemsThe analyzer is misaligned with the XYP instrument.Repo

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 8 PN 89-00002-00-072 Rev. C There is a partial clog in the system.Clean the sample probe. Se

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 9Acquisition ProblemsNot enough control microspheres added to the well.Make sure that yo

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 10 PN 89-00002-00-072 Rev. C Bead Detail IrregularitiesUse these tools to assist in diagnosi

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 11Review reports routinely to detect trends.Use system xMAP control microspheres to chec

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 12 PN 89-00002-00-072 Rev. C Symptom Possible problem SolutionCalibration microspheres clas

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 13Error States The following error states display on the Status Bar on the Run Batch tab

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 14 PN 89-00002-00-072 Rev. C Error Message Possible problem SolutionUnknown Diagnostic Error

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 15Sample Error MessagesSample error messages occur when there is a problem with sample a

PN 89-00002-00-072 Rev. C 2 - 1 SafetySymbols Please become familiar with the information in this chapter before using the equipment. Do not perform

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 16 PN 89-00002-00-072 Rev. C Verify that the system is functioning properly by running the s

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 17Sample probe height is set incorrectly.Adjust the sample probe height.There is a clog

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 18 PN 89-00002-00-072 Rev. C Luminex SD ProblemsIf the empty sheath fluid container is not r

xMAP Technology TroubleshootingPN 89-00002-00-072 Rev. C 7 - 19Verification  To verify your system from the Maintenance tab:1. Vortex xMAP reagent c

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 20 PN 89-00002-00-072 Rev. C 15. Click OK and wait until the Wash completes. The Device Stat

PN 89-00002-00-072 Rev. C 8 - 1Product NumbersHardware8Product Description Customer NumberRear Air Filter CN-0001-01Bottom Air Filter CN-0002-01Air

Luminex 100 IS User Manual Version 2.3 xMAP Technology8 - 2 PN 89-00002-00-072 Rev. C Luminex 100 IS Documentation, Version 2.3 (Manuals on CD)Lumine

xMAP Technology Product NumbersPN 89-00002-00-072 Rev. C 8 - 3SoftwarexMAP ReagentsTrainingSyringe Seal (Qty 4) CN-0014-01Cable, USB CN-0018-01Waste

Luminex 100 IS User Manual Version 2.3 xMAP Technology8 - 4 PN 89-00002-00-072 Rev. C

PN 89-00002-00-072 Rev. C A - 1Glossaryagglutination The coalescing of small particles that are suspended in solution; these larger masses are then

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 2 PN 89-00002-00-072 Rev. C Warnings and Notes Informational notes and warnings appear in t

A - 2 PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyCL1 Refers to dyes embedded in the microsphere. Also see classi

PN 89-00002-00-072 Rev. C A - 3xMAP Technology Luminex xMAP microsphere setLuminex xMAP microsphere setLuminex multi-analyte microspheres containing

A - 4 PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologysample The mixture of assay components (microspheres, reporter,

PN 89-00002-00-072 Rev. C B - 1Luminex 100 IS System InstallationOverview This appendix provides hardware, firmware, and software installation proce

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 2 PN 89-00002-00-072 Rev. C The following sections describe how to connect the Luminex 100

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-072 Rev. C B - 3Connect the Luminex 100 analyzer and Luminex XYP to the PCYou must set up

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 4 PN 89-00002-00-072 Rev. C Figure B-3 Install Alignment Guide7. Ensure that the power sw

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-072 Rev. C B - 5Figure B-4 Luminex 100 IS Connections (Luminex 100 analyzer, Luminex XYP

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 6 PN 89-00002-00-072 Rev. C 12. Connect the color-coded tubing connectors to the Luminex 10

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-072 Rev. C B - 73. Unscrew the cap from the Cheminert fitting. 4. Insert the sample probe

xMAP Technology SafetyPN 89-00002-00-072 Rev. C 2 - 3Do not perform any maintenance or cleaning of the system’s electrical components, with the exce

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 8 PN 89-00002-00-072 Rev. C 7. Plug the Luminex XYP instrument and the Luminex 100 analyzer

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-072 Rev. C B - 92. In a 96-well microtiter plate where overall height is no more than 0.75

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 10 PN 89-00002-00-072 Rev. C  To install the Luminex XYP instrument reservoir:1. On the Ma

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-072 Rev. C B - 113. Place the Luminex SD system near the sheath fluid connection on the le

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 12 PN 89-00002-00-072 Rev. C • Lower the stainless steel filter end of the sheath fluid lin

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-072 Rev. C B - 13Figure B-13 Luminex 100 Analyzer Regulator Adjustment6. From the Home o

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 14 PN 89-00002-00-072 Rev. C  To install the Luminex XYP instrument heater block:1. On the

xMAP Technology Luminex 100 IS 2.3 Software InstallationPN 89-00002-00-072 Rev. C B - 15Luminex 100 Version 1.7 with Windows 98 to Luminex 100 IS Ve

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 16 PN 89-00002-00-072 Rev. C Figure B-15 Luminex 100 IS 2.3 Startup Splash Screen4. At th

xMAP Technology Luminex 100 IS 2.3 Software InstallationPN 89-00002-00-072 Rev. C B - 17Figure B-16 Software Information Window3. Run system calib

© LUMINEX CORPORATION, 2001-2005. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 4 PN 89-00002-00-072 Rev. C The following label appears on the back of the Luminex XYP inst

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 18 PN 89-00002-00-072 Rev. C 4. The InstallShield Wizard—Welcome dialog box opens. See Figu

xMAP Technology Luminex 100 IS 2.3 Software InstallationPN 89-00002-00-072 Rev. C B - 195. Click OK in the Confirmation File Deletion dialog box. If

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 20 PN 89-00002-00-072 Rev. C 2. Choose the file name and location of the database that you

xMAP Technology Luminex 100 IS 2.3 Firmware InstallationPN 89-00002-00-072 Rev. C B - 21Luminex 100 IS 2.3 Firmware InstallationThe Luminex 100 anal

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 22 PN 89-00002-00-072 Rev. C • Luminex XYP instrument—Continue to use the serial interface

xMAP Technology Luminex 100 IS 2.3 Firmware InstallationPN 89-00002-00-072 Rev. C B - 23Figure B-19 LXFirmware Update Tool Dialog Box4. Click Upgr

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 24 PN 89-00002-00-072 Rev. C Update InterfaceCable• If you are upgrading from Luminex 100 V

xMAP Technology Luminex 100 IS 2.3 Firmware InstallationPN 89-00002-00-072 Rev. C B - 25version listed in the upgrade document. Your upgrade kit ins

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 26 PN 89-00002-00-072 Rev. C Verify SuccessfulFirmware Upgrade1. On the PC desktop click St

xMAP Technology Installation DrawingPN 89-00002-00-072 Rev. C B - 27Isometric View Right Side ViewLuminex SD19.55 lb. (8.89 kg.)Luminex XYP32.05 lb.

xMAP Technology SafetyPN 89-00002-00-072 Rev. C 2 - 5You must monitor waste levels manually. Do not allow the waste container to overflow! Empty the

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 28 PN 89-00002-00-072 Rev. C

PN 89-00002-00-072 Rev. C C - 1Output.CSVOverview This chapter describes the file specification for the Luminex 100 IS 2.3 OUTPUT.CSV file. Although

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 2 PN 89-00002-00-072 Rev. C Sample2...SampleMStatNStatN Header: Sample Location, Sample Na

xMAP TechnologyPN 89-00002-00-072 Rev. C C - 3TemplateName, "value” (Optional) The name of the template used to create the batch. Limited to 3

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 4 PN 89-00002-00-072 Rev. C Statistics Definitions Statistical calculations are performed f

xMAP TechnologyPN 89-00002-00-072 Rev. C C - 5Statistics Column DefinitionsThe blocks of statistical data are displayed such that the first row of d

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 6 PN 89-00002-00-072 Rev. C Total Events The number of events that fell with in the defined

xMAP TechnologyPN 89-00002-00-072 Rev. C C - 7Luminex 100 IS OUTPUT.CSV file with no additional features enabled "Program","Luminex 1

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 8 PN 89-00002-00-072 Rev. C Luminex 100 IS OUTPUT.CSV file with all additional features ena

xMAP TechnologyPN 89-00002-00-072 Rev. C C - 9"Samples","8","Min Events","0""Results""DataTyp

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 6 PN 89-00002-00-072 Rev. C All laser apertures are located within the Luminex 100 analyzer

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 10 PN 89-00002-00-072 Rev. C "3","Std l","4450.92","75&q

xMAP TechnologyPN 89-00002-00-072 Rev. C C - 11"1","Std s","69","75","""2","Std m&q

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 12 PN 89-00002-00-072 Rev. C "DataType:","Trimmed StdDev""Location

PN 89-00002-00-072 Rev. C Index-1Aaccess doors 2-7, 3-10accessoriessee accessory instructions 6-11accuracy/precisionspecifications 3-4acquisition ar

Index-2 PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologymeasure 4-1soluble analytes 4-1background samples 5-73barcode

PN 89-00002-00-072 Rev. C Index-3xMAP Technology Indexreports 5-108databasebackup 5-112compressed patient reports 4-3management 5-112, 5-113restore

Index-4 PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologyhelp menu 5-3, 5-119learning about the device 5-117learning a

PN 89-00002-00-072 Rev. C Index-5xMAP Technology Indexsemi-annual 6-6weekly 6-4manual adjustment 3-12Manual recalculate 5-94marketing 2-3Maximize/Mi

Index-6 PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyQQualitative analysis 5-85Quality Control Report 5-108Quantit

PN 89-00002-00-072 Rev. C Index-7xMAP Technology Indexfilter 6-10levels 6-1product number 8-3sheath fluid container 3-12show bead 5-45Shut Down the

xMAP Technology SafetyPN 89-00002-00-072 Rev. C 2 - 7Barcode Reader Laser This label is attached to the barcode reader.Figure 2-9 Barcode Reader La

Index-8 PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologytest rate 4-1testing 5-83testing clinical samples 3-2tests 4-

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 8 PN 89-00002-00-072 Rev. C HeatBlue Indicator LightThe blue light above the Luminex 100 an

xMAP Technology SafetyPN 89-00002-00-072 Rev. C 2 - 9Decontaminating the Luminex 100 Analyzer for Return ShipmentLuminex Technical Support will give

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 10 PN 89-00002-00-072 Rev. C

PN 89-00002-00-072 Rev. C 3 - 1The SystemTheory of Operation Luminex 100 IS technology is based on flow cell fluorometry with Luminex-developed inno

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 2 PN 89-00002-00-072 Rev. C of pressurized sheath fluid. A single 20 liter sheath container

xMAP Technology The SystemPN 89-00002-00-072 Rev. C 3 - 3xMAP Technology Reagents• Classification calibration microspheres (CAL1)• Reporter calibrat

Standard Terms and Conditions For Use of ProductBy opening the packaging containing this product ("Product") or by using such Product in any

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 4 PN 89-00002-00-072 Rev. C acquiring sample, running system calibrators, running system con

xMAP Technology The SystemPN 89-00002-00-072 Rev. C 3 - 555°C (95°F to 131°F) when performing heated assays and using the heater blockLuminex 100 An

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 6 PN 89-00002-00-072 Rev. C Luminex XYP Instrument General• Ambient temperature: 15°C to 30°

xMAP Technology The SystemPN 89-00002-00-072 Rev. C 3 - 7Recommended Additional Equipment UninterruptiblePower Supply(UPS)Luminex highly recommends

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 8 PN 89-00002-00-072 Rev. C Luminex XYPInstrumentVentilation FilterThe XYP instrument ventil

xMAP Technology The SystemPN 89-00002-00-072 Rev. C 3 - 9The alignment guide directs the sample probe into the Luminex XYP instrument.1. Cheminert F

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 10 PN 89-00002-00-072 Rev. C Air Intake Filter A replaceable air intake filter cleans the ai

xMAP Technology The SystemPN 89-00002-00-072 Rev. C 3 - 11Sheath Filter The sheath filter removes particles greater than ten microns in diameter fro

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 12 PN 89-00002-00-072 Rev. C Luminex SheathDelivery SystemFor proper operation, place the L

PN 89-00002-00-072 Rev. C 4 - 1Basic ConceptsBackground InformationxMAP technology is a versatile system that measures soluble analytes. The Luminex

BUYER HEREBY GRANTS TO LUMINEX A NONEXCLUSIVE, WORLDWIDE, UNRE-STRICTED, ROYALTY-FREE, FULLY PAID-UP LICENSE, WITH THE RIGHT TO GRANT AND AUTHORIZE SU

Luminex 100 IS User Manual Version 2.3 xMAP Technology4 - 2 PN 89-00002-00-072 Rev. C Photodiodes and a photomultiplier tube receive fluorescent sign

xMAP Technology Basic ConceptsPN 89-00002-00-072 Rev. C 4 - 3System calibrators and controls are provided as part of the system. Calibrate at least

Luminex 100 IS User Manual Version 2.3 xMAP Technology4 - 4 PN 89-00002-00-072 Rev. C

PN 89-00002-00-072 Rev. C 5 - 1Using Luminex 100 IS 2.3 SoftwareThis manual describes how to use the Luminex IS 2.3 software. It includes a glossary

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 2 PN 89-00002-00-072 Rev. C .Figure 5-1. Luminex IS 2.3 Main WindowThere are five major pa

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 3Menu Bar The menu bar contains the following menus: File Menu, Tools

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 4 PN 89-00002-00-072 Rev. C Toolbar The shortcut buttons on the toolbar can be found in oth

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 5Favorites. You can use the Favorites list to create a customized lis

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 6 PN 89-00002-00-072 Rev. C 1. Microtiter plate/reservoir image 2. Temperature and Pressur

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 7The DD temperature measures the doublet discriminator temperature. D

End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 8 PN 89-00002-00-072 Rev. C Maintenance Tab Use the Maintenance tab to perform maintenance,

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 9Instructions for maintenance procedures begin on page 5-93. Table 5-

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 10 PN 89-00002-00-072 Rev. C The system diagnoses real-time system problems related to flui

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 11The Detailed Sample Progress section displays the percentage of com

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 12 PN 89-00002-00-072 Rev. C Figure 5-10. Message LogThe log displays actions in color-cod

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 13Acquisition Detail tab features:• Batch Name and Description• Batch

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 14 PN 89-00002-00-072 Rev. C The Batch Name and Description section displays the name of th

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 15 1. Gate Boundaries 2. Aggregate Beads 3. Numerical Gate Position

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 16 PN 89-00002-00-072 Rev. C Figure 5-14. Dot Plot Display ExampleFour buttons appear at t

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 17collected events. Post acquisition does not display decaying dot pl

5. EXPORT RESTRICTIONS. You agree that you will not export or re-export the SOFTWARE PRODUCT to any country, person, entity, or end-user subject to U.

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 18 PN 89-00002-00-072 Rev. C The Status Bar displays status information as the software pro

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 19Table 5-4. Status BarCommunication Message DetailsStatus Bar Color

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 20 PN 89-00002-00-072 Rev. C Secondary WindowsIn addition to the main window, there are oth

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 21• Command list• Microtiter Plate image• Command button group box• S

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 22 PN 89-00002-00-072 Rev. C The New Lot button is visible only if the template you are usi

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 23controls, and unknown samples are not always acquired in sequential

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 24 PN 89-00002-00-072 Rev. C Standards Tab The Standards tab lists all the batch tests with

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 25Figure 5-17. Analysis Window - Standards Tab OpenWhen a median flu

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 26 PN 89-00002-00-072 Rev. C Figure 5-18. Non-Competitive AssayExamples of out-of-range sa

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 27Figure 5-19. Competitive AssayThe standards tab displays an Expect

PN 89-00002-00-072 Rev. C iIntroduction 1-1About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1The Luminex

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 28 PN 89-00002-00-072 Rev. C than one background sample, the fluorescence values of the two

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 29Figure 5-20. Analysis Window - Samples Tab OpenReplicateAveragingS

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 30 PN 89-00002-00-072 Rev. C If the system fails to determine the results for a replicate d

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 31Commands This section describes the different commands used in the

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 32 PN 89-00002-00-072 Rev. C Connect to Instrument Establishes a connection between the PC

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 33Export Data Exports data to a .csv file in the batch folder. There

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 34 PN 89-00002-00-072 Rev. C New Advanced Batch Assay developers use New Advanced Batch to

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 35Previous Test This command is found in the Analysis window. Use thi

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 36 PN 89-00002-00-072 Rev. C command. However, the sanitize command uses 10% to 20% househo

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 37Start Analysis Opens the Open Batch dialog box, from which you sel

ii PN 89-00002-00-072 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyElectronics . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 38 PN 89-00002-00-072 Rev. C Procedures This section explains how to perform functions in t

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 39Setting Software Options Set up and customize the system software a

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 40 PN 89-00002-00-072 Rev. C Auto-Start Analysis. Select this feature to enable the softwar

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 41 Figure 5-22. Options Dialog Box—Company Information TabDefine Dat

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 42 PN 89-00002-00-072 Rev. C Export Location Label Style. Choose one of these options to d

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 432. Select the command that you want to add to your Favorites list.

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 44 PN 89-00002-00-072 Rev. C When priming, the system draws Luminex xMAP sheath fluid from

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 45distilled water after Sanitize. Place at least 200 µL in a microtit

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 46 PN 89-00002-00-072 Rev. C  To set the Luminex XYP instrument heater temperature:1. Clic

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 47Ensure that the Luminex analyzer lasers are warmed up and the probe

PN 89-00002-00-072 Rev. C iiixMAP Technology ContentsStatus Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-17S

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 48 PN 89-00002-00-072 Rev. C Figure 5-25. Update CAL Targets Dialog Box10. Repeat step 9

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 49You must run system controls following calibration. Continue with t

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 50 PN 89-00002-00-072 Rev. C 4. Ensure that the analyzer is set to draw CON1 and CON2 beads

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 512. Click Import CAL or Import CON as appropriate. The Open dialog b

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 52 PN 89-00002-00-072 Rev. C You can group batches together as a multi-batch. Multi-batches

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 53 To import a template from a diskette or CD:1. Insert the kit’s di

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 54 PN 89-00002-00-072 Rev. C 5. If you want to insert an Acquire Patient or Skip command, s

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 55If all patient IDs in the batch are identified, the system appends

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 56 PN 89-00002-00-072 Rev. C 2. Click Clear in the dialog box.3. Click Yes to confirm that

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-072 Rev. C 5 - 57 To reprocess samples using Replay Batch:1. On the Acq. Detail tab