Luminex 100 IS User Manual, Version 2.3 Manuale Utente

Luminex 100™ IS User Manual Version 2.3 Lum/nex®

iv PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyMaximize/Minimize. . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 58 PN 89-00002-00-071 Rev. C 2. Select the unprocessed batch that you want to delete. 3. Cl

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 593. Create a New Batch. The procedure is shown on page 5-53. Click S

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 60 PN 89-00002-00-071 Rev. C  To open a multi-batch:1. Click Open Multi-Batch. The Open Mu

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 612. Insert the first plate.3. On the Run Batch tab, click Start Plat

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 62 PN 89-00002-00-071 Rev. C 2. Squeeze the barcode reader’s trigger. The beam activates an

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 63 To add a Patient List file while creating a batch or multi-batch:

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 64 PN 89-00002-00-071 Rev. C  To edit a patient list:1. On the File menu, select Edit Pati

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 653. Select the item that uses the dilution factor that you are setti

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 66 PN 89-00002-00-071 Rev. C  To create a New Advanced Batch:1. Open the Acq. Detail tab.

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 676. Click the Bead Set tab. Select the checkboxes next to each desir

PN 89-00002-00-071 Rev. C vxMAP Technology ContentsDefine Data Export Tab Information. . . . . . . . . . . . . . . .5-41Setting up the Favorites Lis

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 68 PN 89-00002-00-071 Rev. C define commands that apply to one or more wells, one or more r

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 69Figure 5-38. Plate Layout Tab Command Menu11. Establish preliminar

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 70 PN 89-00002-00-071 Rev. C Figure 5-40. Plate Layout Tab, Off-Plate Commands12. Insert o

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 71Managing Assay Lots You can edit standard and control lot informati

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 72 PN 89-00002-00-071 Rev. C tinue. Once you enter the numbers, the Update Lot Informa-tion

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 734. Enter the expiration date in the Exp. Date field.5. Enter the st

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 74 PN 89-00002-00-071 Rev. C 4. Click Save. The system updates the lot changes and applies

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 75 Figure 5-45. Update Lot Information Dialog BoxExport a Lot froman

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 76 PN 89-00002-00-071 Rev. C After responding to the confirmation dialog boxes, regarding s

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 77curve. Replicate samples are averaged to determine the reported qua

vi PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyDatabase Management Procedures . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 78 PN 89-00002-00-071 Rev. C Customization Dialog Box Buttons:OK: click to update the graph

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 79are included in the graph, then Monochrome with Symbols will help d

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 80 PN 89-00002-00-071 Rev. C Figure 5-49. Customization Dialog Box—Font Tab• Color Tab—Us

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 81 Figure 5-50. Customization Dialog Box—Color Tab• Style Tab—use th

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 82 PN 89-00002-00-071 Rev. C Figure 5-52. Graph Menu ItemsEnable AutomaticAnalysisYou can

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 832. Click the Auto-Start Analysis checkbox, then click OK. When the

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 84 PN 89-00002-00-071 Rev. C Invalidating standards greatly affects the curve fit and subse

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 853. The appropriate Standard or Control dialog box opens. To invalid

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 86 PN 89-00002-00-071 Rev. C Figure 5-56. Choose Lot Dialog Box—Standard Only3. To create

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 87Maintenance Report - provides a history of all maintenance operatio

PN 89-00002-00-071 Rev. C viixMAP Technology ContentsPressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 88 PN 89-00002-00-071 Rev. C Figure 5-58. Batch Selection Dialog Box3. Enter the informati

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 89Export Batch Data  To export batch data:1. On the File menu, , cli

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 90 PN 89-00002-00-071 Rev. C 5. At the Microsoft Windows Print window, select your printer,

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 91 To back up the database:1. On the Tools menu, click Database Back

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 92 PN 89-00002-00-071 Rev. C 3. From the Restore Database From dialog box, select a databas

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 93Drain the Analyzer When draining, you do not need to supply solutio

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 94 PN 89-00002-00-071 Rev. C  To drain the system:1. On the Maintenance tab, click Eject/R

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 95 Figure 5-62. Cleanup Utility Dialog Box To perform a disk cleanu

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 96 PN 89-00002-00-071 Rev. C  To delete the batch folders:1. In the Cleanup Utility dialog

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 97Perform a SoakCommandSoak the sample probe to prevent the sheath fl

viii PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyArchive “My Sessions” folder. . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 98 PN 89-00002-00-071 Rev. C

PN 89-00002-00-071 Rev. C 6 - 1Maintenance and CleaningTo ensure accurate test results, properly clean and maintain the Luminex 100 IS system. Read

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 2 PN 89-00002-00-071 Rev. C 5. Click Wash. In the confirmation dialog box, click on the dro

xMAP Technology Maintenance and CleaningPN 89-00002-00-071 Rev. C 6 - 3Refill the SheathFluid Container To refill the sheath fluid container:1. Rel

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 4 PN 89-00002-00-071 Rev. C Flush the System Run 3 backflushes, 3 drains, 2 alcohol flushes

xMAP Technology Maintenance and CleaningPN 89-00002-00-071 Rev. C 6 - 53. Open both doors of the analyzer and clean all accessible surfaces with det

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 6 PN 89-00002-00-071 Rev. C Figure 6-2 Grasping the Tubing4. Remove the filter with one ha

xMAP Technology Maintenance and CleaningPN 89-00002-00-071 Rev. C 6 - 7 Figure 6-3 Removing the Screen3. Replace the filter and replace the screen.

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 8 PN 89-00002-00-071 Rev. C Syringe Seal  To replace the syringe plunger seal:1. Disconnec

xMAP Technology Maintenance and CleaningPN 89-00002-00-071 Rev. C 6 - 911. Hand-tighten the screw on the syringe arm.12. Plug in the power cord and

PN 89-00002-00-071 Rev. C 1 - 1IntroductionAbout This Manual This manual provides you with information to understand and use the Luminex® 100™ IS sy

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 10 PN 89-00002-00-071 Rev. C AnnuallySheath Filter  To change the Luminex 100 analyzer she

xMAP Technology Maintenance and CleaningPN 89-00002-00-071 Rev. C 6 - 11As requiredFuses To replace the fuses in either the Luminex 100 analyzer or

Luminex 100 IS User Manual Version 2.3 xMAP Technology6 - 12 PN 89-00002-00-071 Rev. C

Luminex 100 IS Maintenance Log Month(s): ________________________________________Year:_______________Maintenance LogProcedure

PN 89-00002-00-071 Rev. C 7 - 1 TroubleshootingTroubleshooting the Luminex 100 IS SystemTroubleshooting procedures help users isolate, identify, and

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 2 PN 89-00002-00-071 Rev. C 7:00 a.m. and 7:00 p.m. Central Time, Monday through Friday. Inq

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 3Pressurization Normal air and sheath pressure readings vary between 6-9 psi while the c

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 4 PN 89-00002-00-071 Rev. C Fluid Leaks Fluid leaks can result in poor pressurization and fa

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 5Sample Probe Problems with the sample probe can lead to fluid leaks and pressurization

Luminex 100 IS User Manual Version 2.3 xMAP Technology1 - 2 PN 89-00002-00-071 Rev. C Technical Support Luminex Technical Support representatives are

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 6 PN 89-00002-00-071 Rev. C Sample arm stuck in the down position.The sample probe height is

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 7Calibration and Control ProblemsThe analyzer is misaligned with the XYP instrument.Repo

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 8 PN 89-00002-00-071 Rev. C There is a partial clog in the system.Clean the sample probe. Se

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 9Acquisition ProblemsNot enough control microspheres added to the well.Make sure that yo

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 10 PN 89-00002-00-071 Rev. C Bead Detail IrregularitiesUse these tools to assist in diagnosi

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 11• error messagesReview reports routinely to detect trends.Use system xMAP control micr

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 12 PN 89-00002-00-071 Rev. C Symptom Possible problem SolutionCalibration microspheres clas

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 13Error States The following error states display on the Status Bar on the Run Batch tab

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 14 PN 89-00002-00-071 Rev. C Error Message Possible problem SolutionUnknown Diagnostic Error

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 15Sample Error MessagesSample error messages occur when there is a problem with sample a

PN 89-00002-00-071 Rev. C 2 - 1 SafetySymbols Please become familiar with the information in this chapter before using the equipment. Do not perform

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 16 PN 89-00002-00-071 Rev. C Verify that the system is functioning properly by running the s

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 17Sample probe height is set incorrectly.Adjust the sample probe height.There is a clog

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 18 PN 89-00002-00-071 Rev. C Luminex SD ProblemsIf the empty sheath fluid container is not r

xMAP Technology TroubleshootingPN 89-00002-00-071 Rev. C 7 - 19Verification  To verify your system from the Maintenance tab:1. Vortex xMAP reagent c

Luminex 100 IS User Manual Version 2.3 xMAP Technology7 - 20 PN 89-00002-00-071 Rev. C 15. Click OK and wait until the Wash completes. The Device Stat

PN 89-00002-00-071 Rev. C 8 - 1Product NumbersHardware8Product Description Customer NumberRear Air Filter CN-0001-01Bottom Air Filter CN-0002-01Air

Luminex 100 IS User Manual Version 2.3 xMAP Technology8 - 2 PN 89-00002-00-071 Rev. C Luminex 100 IS Documentation Version 2.3 (Manuals on CD)Luminex

xMAP Technology Product NumbersPN 89-00002-00-071 Rev. C 8 - 3SoftwarexMAP ReagentsTrainingSyringe Seal (Qty 4) CN-0014-01Cable, USB CN-0018-01Waste

Luminex 100 IS User Manual Version 2.3 xMAP Technology8 - 4 PN 89-00002-00-071 Rev. C

PN 89-00002-00-071 Rev. C A - 1Glossaryagglutination The coalescing of small particles that are suspended in solution; these larger masses are then

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 2 PN 89-00002-00-071 Rev. C Warnings and Notes Informational notes and warnings appear in t

A - 2 PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyCL1 Refers to dyes embedded in the microsphere. Also see classi

PN 89-00002-00-071 Rev. C A - 3xMAP Technology Luminex xMAP microsphere setLuminex xMAP microsphere setLuminex multi-analyte microspheres containing

A - 4 PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologysample The mixture of assay components (microspheres, reporter,

PN 89-00002-00-071 Rev. C B - 1Luminex 100 IS System InstallationOverview This appendix provides hardware, firmware, and software installation proce

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 2 PN 89-00002-00-071 Rev. C The following sections describe how to connect the Luminex 100

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-071 Rev. C B - 3Connect the Luminex 100 analyzer and Luminex XYP to the PCYou must set up

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 4 PN 89-00002-00-071 Rev. C Figure B-3 Install Alignment Guide7. Ensure that the power sw

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-071 Rev. C B - 5Figure B-4 Luminex 100 IS Connections (Luminex 100 analyzer, Luminex XYP

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 6 PN 89-00002-00-071 Rev. C 12. Connect the color-coded tubing connectors to the Luminex 10

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-071 Rev. C B - 73. Unscrew the cap from the Cheminert fitting. 4. Insert the sample probe

xMAP Technology SafetyPN 89-00002-00-071 Rev. C 2 - 3Do not perform any maintenance or cleaning of the system’s electrical components, with the exce

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 8 PN 89-00002-00-071 Rev. C 7. Plug the Luminex XYP instrument and the Luminex 100 analyzer

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-071 Rev. C B - 92. In a 96-well microtiter plate where overall height is no more than 0.75

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 10 PN 89-00002-00-071 Rev. C  To install the Luminex XYP instrument reservoir:1. On the Ma

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-071 Rev. C B - 113. Place the Luminex SD system near the sheath fluid connection on the le

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 12 PN 89-00002-00-071 Rev. C • Lower the stainless steel filter end of the sheath fluid lin

xMAP Technology Luminex 100 IS System SetupPN 89-00002-00-071 Rev. C B - 13Figure B-13 Luminex 100 Analyzer Regulator Adjustment6. From the Home o

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 14 PN 89-00002-00-071 Rev. C  To install the Luminex XYP instrument heater block:1. On the

xMAP Technology Luminex 100 IS 2.3 Software InstallationPN 89-00002-00-071 Rev. C B - 15Luminex 100 Version 1.7 with Windows 98 to Luminex 100 IS Ve

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 16 PN 89-00002-00-071 Rev. C Figure B-15 Luminex 100 IS 2.3 Startup Splash Screen4. At th

xMAP Technology Luminex 100 IS 2.3 Software InstallationPN 89-00002-00-071 Rev. C B - 17Figure B-16 Software Information Window3. Run system calib

© LUMINEX CORPORATION, 2001-2005. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 4 PN 89-00002-00-071 Rev. C The following label appears on the back of the Luminex XYP inst

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 18 PN 89-00002-00-071 Rev. C 4. The InstallShield Wizard—Welcome dialog box opens. See Figu

xMAP Technology Luminex 100 IS 2.3 Software InstallationPN 89-00002-00-071 Rev. C B - 195. Click OK in the Confirmation File Deletion dialog box. If

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 20 PN 89-00002-00-071 Rev. C 2. Choose the file name and location of the database that you

xMAP Technology Luminex 100 IS 2.3 Firmware InstallationPN 89-00002-00-071 Rev. C B - 21Luminex 100 IS 2.3 Firmware InstallationThe Luminex 100 anal

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 22 PN 89-00002-00-071 Rev. C • Luminex XYP instrument—Continue to use the serial interface

xMAP Technology Luminex 100 IS 2.3 Firmware InstallationPN 89-00002-00-071 Rev. C B - 23Figure B-19 LXFirmware Update Tool Dialog Box4. Click Upgr

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 24 PN 89-00002-00-071 Rev. C • If you are upgrading from Luminex 100 IS Version 2.1 or 2.2

xMAP Technology Luminex 100 IS 2.3 Firmware InstallationPN 89-00002-00-071 Rev. C B - 25Luminex SD System Firmware UpdateIf you are upgrading the fi

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 26 PN 89-00002-00-071 Rev. C 3. Turn the power off to the SD, XYP, and LX100. 4. Reconnect

xMAP Technology Installation DrawingPN 89-00002-00-071 Rev. C B - 27Isometric View Right Side ViewLuminex SD19.55 lb. (8.89 kg.)Luminex XYP32.05 lb.

xMAP Technology SafetyPN 89-00002-00-071 Rev. C 2 - 5You must monitor waste levels manually. Do not allow the waste container to overflow! Empty the

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyB - 28 PN 89-00002-00-071 Rev. C

PN 89-00002-00-071 Rev. C C - 1Output.CSVOverview This chapter describes the file specification for the Luminex 100 IS 2.3 OUTPUT.CSV file. Although

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 2 PN 89-00002-00-071 Rev. C Sample2...SampleMStatNStatN Header: Sample Location, Sample Na

xMAP TechnologyPN 89-00002-00-071 Rev. C C - 3TemplateName, "value” (Optional) The name of the template used to create the batch. Limited to 3

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 4 PN 89-00002-00-071 Rev. C Statistics Definitions Statistical calculations are performed f

xMAP TechnologyPN 89-00002-00-071 Rev. C C - 5Statistics Column DefinitionsThe blocks of statistical data are displayed such that the first row of d

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 6 PN 89-00002-00-071 Rev. C Total Events The number of events that fell with in the defined

xMAP TechnologyPN 89-00002-00-071 Rev. C C - 7Luminex 100 IS OUTPUT.CSV file with no additional features enabled "Program","Luminex 1

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 8 PN 89-00002-00-071 Rev. C Luminex 100 IS OUTPUT.CSV file with all additional features ena

xMAP TechnologyPN 89-00002-00-071 Rev. C C - 9"Samples","8","Min Events","0""Results""DataTyp

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 6 PN 89-00002-00-071 Rev. C All laser apertures are located within the Luminex 100 analyzer

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 10 PN 89-00002-00-071 Rev. C "3","Std l","4450.92","75&q

xMAP TechnologyPN 89-00002-00-071 Rev. C C - 11"1","Std s","69","75","""2","Std m&q

Luminex 100 IS User Manual Version 2.3 xMAP TechnologyC - 12 PN 89-00002-00-071 Rev. C "DataType:","Trimmed StdDev""Location

PN 89-00002-00-071 Rev. C Index-1Aaccess doors 2-7, 3-9accessoriessee accessory instructions 6-11accuracy/precisionspecifications 3-4add sheath flui

Index-2 PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologycleanaccessible surfaces 6-4Clear Preliminary Off-plate comma

PN 89-00002-00-071 Rev. C Index-3xMAP Technology Indexhigh waste volume 6-3Iidlemore than 4 hours 6-1improper system operation 3-3insert off-plate c

Index-4 PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologyphotodiode 4-1photomultiplier 4-1plate 4-1plate holderejectin

PN 89-00002-00-071 Rev. C Index-5xMAP Technology Indexsheath fluid container 3-11software 3-3, 8-3software overviewLuminex 100 IS software 4-2softwa

Index-6 PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP Technologywarranty 2-2wasteconnector 3-11container 3-12, 6-2high volume

xMAP Technology SafetyPN 89-00002-00-071 Rev. C 2 - 7Barcode Reader Laser This label is attached to the barcode reader.Figure 2-9 Barcode Reader La

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 8 PN 89-00002-00-071 Rev. C HeatBlue Indicator LightThe blue light above the Luminex 100 an

xMAP Technology SafetyPN 89-00002-00-071 Rev. C 2 - 9Decontaminating the Luminex 100 Analyzer for Return ShipmentLuminex Technical Support will give

Luminex 100 IS User Manual Version 2.3 xMAP Technology2 - 10 PN 89-00002-00-071 Rev. C

PN 89-00002-00-071 Rev. C 3 - 1The SystemTheory of Operation Luminex 100 IS technology is based on flow cell fluorometry with Luminex-developed inno

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 2 PN 89-00002-00-071 Rev. C of pressurized sheath fluid. A single 20 liter sheath container

xMAP Technology The SystemPN 89-00002-00-071 Rev. C 3 - 3xMAP Technology Reagents• Classification calibration microspheres (CAL1)• Reporter calibrat

Standard Terms and Conditions For Use of ProductBy opening the packaging containing this product ("Product") or by using such Product in any

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 4 PN 89-00002-00-071 Rev. C acquiring sample, running system calibrators, running system con

xMAP Technology The SystemPN 89-00002-00-071 Rev. C 3 - 555°C (95°F to 131°F) when performing heated assays and using the heater blockLuminex 100 An

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 6 PN 89-00002-00-071 Rev. C Luminex XYP Instrument General• Ambient temperature: 15°C to 30°

xMAP Technology The SystemPN 89-00002-00-071 Rev. C 3 - 7Recommended Additional Equipment UninterruptiblePower Supply(UPS)Luminex highly recommends

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 8 PN 89-00002-00-071 Rev. C Luminex XYPInstrumentVentilation FilterThe XYP instrument ventil

xMAP Technology The SystemPN 89-00002-00-071 Rev. C 3 - 9The alignment guide directs the sample probe into the Luminex XYP instrument.1. Cheminert F

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 10 PN 89-00002-00-071 Rev. C Air Intake Filter A replaceable air intake filter cleans the ai

xMAP Technology The SystemPN 89-00002-00-071 Rev. C 3 - 11Sheath Filter The sheath filter removes particles greater than ten microns in diameter fro

Luminex 100 IS User Manual Version 2.3 xMAP Technology3 - 12 PN 89-00002-00-071 Rev. C Luminex SheathDelivery SystemFor proper operation, place the L

PN 89-00002-00-071 Rev. C 4 - 1Basic ConceptsBackground InformationxMAP technology is a versatile system that measures soluble analytes. The Luminex

BUYER HEREBY GRANTS TO LUMINEX A NONEXCLUSIVE, WORLDWIDE, UNRE-STRICTED, ROYALTY-FREE, FULLY PAID-UP LICENSE, WITH THE RIGHT TO GRANT AND AUTHORIZE SU

Luminex 100 IS User Manual Version 2.3 xMAP Technology4 - 2 PN 89-00002-00-071 Rev. C Photodiodes and a photomultiplier tube receive fluorescent sign

xMAP Technology Basic ConceptsPN 89-00002-00-071 Rev. C 4 - 3System calibrators and controls are provided as part of the system. Calibrate at least

Luminex 100 IS User Manual Version 2.3 xMAP Technology4 - 4 PN 89-00002-00-071 Rev. C

PN 89-00002-00-071 Rev. C 5 - 1Using Luminex 100 IS 2.3 SoftwareThis manual describes how to use the Luminex IS 2.3 software. It includes a glossary

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 2 PN 89-00002-00-071 Rev. C .Figure 5-1. Luminex IS 2.3 Main WindowThere are five major pa

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 3Menu Bar The menu bar contains the following menus: File Menu, Tools

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 4 PN 89-00002-00-071 Rev. C Toolbar The shortcut buttons on the toolbar can be found in oth

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 5Favorites. You can use the Favorites list to create a customized lis

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 6 PN 89-00002-00-071 Rev. C 1. Microtiter plate/reservoir image 2. Temperature and Pressur

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 7The DD temperature measures the doublet discriminator temperature. D

End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 8 PN 89-00002-00-071 Rev. C Maintenance Tab Use the Maintenance tab to perform maintenance,

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 9Instructions for maintenance procedures begin on page 5-92. Table 5-

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 10 PN 89-00002-00-071 Rev. C The system diagnoses real-time system problems related to flui

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 11The Detailed Sample Progress section displays the percentage of com

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 12 PN 89-00002-00-071 Rev. C Figure 5-10. Message LogThe log displays actions in color-cod

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 13Acquisition Detail tab features:• Batch Name and Description• Batch

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 14 PN 89-00002-00-071 Rev. C The Batch Name and Description section displays the name of th

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 15 1. Gate Boundaries 2. Aggregate Beads 3. Numerical Gate Position

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 16 PN 89-00002-00-071 Rev. C Figure 5-14. Dot Plot Display ExampleFour buttons appear at t

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 17collected events. Post acquisition does not display decaying dot pl

5. EXPORT RESTRICTIONS. You agree that you will not export or re-export the SOFTWARE PRODUCT to any country, person, entity, or end-user subject to U.

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 18 PN 89-00002-00-071 Rev. C The Status Bar displays status information as the software pro

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 19Table 5-4. Status BarCommunication Message DetailsStatus Bar Color

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 20 PN 89-00002-00-071 Rev. C Secondary WindowsIn addition to the main window, there are oth

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 21• Microtiter Plate image• Command button group box• Save group box•

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 22 PN 89-00002-00-071 Rev. C The New Lot button is visible only if the template you are usi

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 23controls, and unknown samples are not always acquired in sequential

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 24 PN 89-00002-00-071 Rev. C Standards Tab The Standards tab lists all the batch tests with

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 25Figure 5-17. Analysis Window - Standards Tab OpenWhen a median flu

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 26 PN 89-00002-00-071 Rev. C Figure 5-18. Non-Competitive AssayExamples of out-of-range sa

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 27Figure 5-19. Competitive AssayThe standards tab displays an Expect

PN 89-00002-00-071 Rev. C iIntroduction 1-1About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1The Luminex

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 28 PN 89-00002-00-071 Rev. C than one background sample, the fluorescence values of the two

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 29Figure 5-20. Analysis Window - Samples Tab OpenReplicateAveragingS

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 30 PN 89-00002-00-071 Rev. C If the system fails to determine the results for a replicate d

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 31Commands This section describes the different commands used in the

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 32 PN 89-00002-00-071 Rev. C Connect to Instrument Establishes a connection between the PC

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 33Export Data Exports data to a .csv file in the batch folder. There

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 34 PN 89-00002-00-071 Rev. C New Advanced Batch Assay developers use New Advanced Batch to

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 35Previous Test This command is found in the Analysis window. Use thi

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 36 PN 89-00002-00-071 Rev. C command. However, the sanitize command uses 10% to 20% househo

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 37Start Analysis Opens the Open Batch dialog box, from which you sel

ii PN 89-00002-00-071 Rev. C Luminex 100 IS User Manual Version 2.3 xMAP TechnologyElectronics . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 38 PN 89-00002-00-071 Rev. C Procedures This section explains how to perform functions in t

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 39Setting Software Options Set up and customize the system software a

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 40 PN 89-00002-00-071 Rev. C Auto-Start Analysis. Select this feature to enable the softwar

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 41 Figure 5-22. Options Dialog Box—Company Information TabDefine Dat

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 42 PN 89-00002-00-071 Rev. C Export Location Label Style. Choose one of these options to d

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 432. Select the command that you want to add to your Favorites list.

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 44 PN 89-00002-00-071 Rev. C When priming, the system draws Luminex xMAP sheath fluid from

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 45distilled water after Sanitize. Place at least 200 µL in a microtit

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 46 PN 89-00002-00-071 Rev. C  To set the Luminex XYP instrument heater temperature:1. Clic

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 47Ensure that the Luminex analyzer lasers are warmed up and the probe

PN 89-00002-00-071 Rev. C iiixMAP Technology ContentsStatus Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-17S

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 48 PN 89-00002-00-071 Rev. C Figure 5-25. Update CAL Targets Dialog Box10. Repeat step 9

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 49You must run system controls following calibration. Continue with t

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 50 PN 89-00002-00-071 Rev. C 4. Ensure that the analyzer is set to draw CON1 and CON2 beads

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 512. Click Import CAL or Import CON as appropriate. The Open dialog b

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 52 PN 89-00002-00-071 Rev. C You can group batches together as a multi-batch. Multi-batches

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 53 To import a template from a diskette or CD:1. Insert the kit’s di

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 54 PN 89-00002-00-071 Rev. C 5. If you want to insert an Acquire Patient or Skip command, s

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 55If all patient IDs in the batch are identified, the system appends

Luminex 100 IS User Manual Version 2.3 xMAP Technology5 - 56 PN 89-00002-00-071 Rev. C 2. Click Clear in the dialog box.3. Click Yes to confirm that

xMAP Technology Using Luminex 100 IS 2.3 SoftwarePN 89-00002-00-071 Rev. C 5 - 572. Select the desired batch under the My Batches folder and click O