Luminex 100 IS User Manual, Version 2.1 Manuale Utente

Luminex 100™ IS User Manual Version 2.1Lum/nex® International English Version

vi PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP TechnologyTroubleshooting 9-1Troubleshooting the Luminex 100 IS. . . . . . . .

100 IS S Users Manual Version 2.1 xMAP Technology7 - 36 PN 89-00002-00-070 Rev. A2. Select the template in which you want to apply the new lot and cli

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 37Update LotInformation To update information to an existing lot:1. Click New Lot from the

100 IS S Users Manual Version 2.1 xMAP Technology7 - 38 PN 89-00002-00-070 Rev. AImport Lots ToExistingTemplatesYou may need to import a lot from anot

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 393. Click Export Lot. A Lot Exported dialog box appears confirming the location to which t

100 IS S Users Manual Version 2.1 xMAP Technology7 - 40 PN 89-00002-00-070 Rev. AFigure 74. Luminex Batch Setup Dialog Box4. Enter the batch name (if

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 41Figure 75. Entering Additional Samples7. Enter the sample ID for the sample you want to

100 IS S Users Manual Version 2.1 xMAP Technology7 - 42 PN 89-00002-00-070 Rev. Apatient ID in the list to the unassigned sample acquisition command.

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 43Delete a Batch The system allows you to delete batches that have not been processed.Make

100 IS S Users Manual Version 2.1 xMAP Technology7 - 44 PN 89-00002-00-070 Rev. AFigure 77. Luminex Multi-Batch Setup Dialog Box2. If you want to add

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 456. Select the template that you want to use in your new batch.7. Click the Open button. T

PN 89-00002-00-070 Rev. A viixMAP TechnologyProduct Numbers 10-1Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

100 IS S Users Manual Version 2.1 xMAP Technology7 - 46 PN 89-00002-00-070 Rev. A16. Select a starting location on the microtiter plate if necessary.1

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 47plate appears on the screen to make room for additional samples. The new plate appears to

100 IS S Users Manual Version 2.1 xMAP Technology7 - 48 PN 89-00002-00-070 Rev. AFigure 79. New Plate Added2. Open the batch, as necessary.3. Load th

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 496. Click Resume. The system resumes the acquisition process.Scan in NewSamples with theBa

100 IS S Users Manual Version 2.1 xMAP Technology7 - 50 PN 89-00002-00-070 Rev. A• any other comma delimited values in the same line will be ignored (

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 513. Click Load Patient List. An Open Patient List File dialog box appears.Figure 83. Open

100 IS S Users Manual Version 2.1 xMAP Technology7 - 52 PN 89-00002-00-070 Rev. AEstablish Where toBegin AcquiringDataWhen you are creating a batch or

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 532. Select the template that uses the product for which you are assigning a dilution facto

100 IS S Users Manual Version 2.1 xMAP Technology7 - 54 PN 89-00002-00-070 Rev. Awell for failed acquisitions. Samples may fail analysis for many reas

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 55Selecting Cancel All stops batch acquisition, and you will be able to process it again. T

viii PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP Technology

100 IS S Users Manual Version 2.1 xMAP Technology7 - 56 PN 89-00002-00-070 Rev. A To analyze data from processed batches and multi-batches:1. Click A

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 57Use the F2 key to select the next test or the F3 key to select the previous test in the b

100 IS S Users Manual Version 2.1 xMAP Technology7 - 58 PN 89-00002-00-070 Rev. AErrors Tab The Errors tab shows a list of errors that occurred during

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 59There are six function keys on the standards tab:• F2 - Next Test• F3 - Previous Test• F4

100 IS S Users Manual Version 2.1 xMAP Technology7 - 60 PN 89-00002-00-070 Rev. APerform BatchData Reduction To perform batch data reduction:1. Click

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 61Calibration Trend Report. This report provides information about all calibration operatio

100 IS S Users Manual Version 2.1 xMAP Technology7 - 62 PN 89-00002-00-070 Rev. AFigure 88. Batch Selection Dialog Box3. Enter the information (for e

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 63Figure 90. Report Print Preview Dialog Box6. Click Next until you get to the Finish butt

100 IS S Users Manual Version 2.1 xMAP Technology7 - 64 PN 89-00002-00-070 Rev. AFigure 92. Batch Data Exported Dialog Box4. Click the OK button.Data

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 65When the database is 98% full, sample acquisition is prevented. System calibration and co

PN 89-00002-00-070 Rev. A 1 - 1IntroductionAbout This Manual This manual introduces you to the system, and with step-by-step instructions, it guides y

100 IS S Users Manual Version 2.1 xMAP Technology7 - 66 PN 89-00002-00-070 Rev. AEdit Menu Information from batches may be copied into other documents

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 67 To clear the message log:1. Right-click the information with your mouse and drag to Cle

100 IS S Users Manual Version 2.1 xMAP Technology7 - 68 PN 89-00002-00-070 Rev. AFigure 95. About Device Dialog Box3. Click the OK button to close th

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 69Figure 96. About IS Software Dialog Box3. Click the OK button.Shut Down the Luminex 100

100 IS S Users Manual Version 2.1 xMAP Technology7 - 70 PN 89-00002-00-070 Rev. A5. Click Soak to fill the Sample Probe and sample lines with distille

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 71Exit the Luminex 100 IS SoftwareIf you try to exit the system for any reason, a dialog bo

100 IS S Users Manual Version 2.1 xMAP Technology7 - 72 PN 89-00002-00-070 Rev. A

PN 89-00002-00-070 Rev. A 8 - 1Maintenance and CleaningProper maintenance and cleaning of the Luminex 100 IS is required Read and follow all instructi

100 IS User Manual Version 2.1 xMAP Technology8 - 2 PN 89-00002-00-070 Rev. AAfter Running Samples  After running samples, follow these steps:1. San

xMAP Technology Maintenance and CleaningPN 89-00002-00-070 Rev. A 8 - 32. Wipe all exterior surfaces with mild detergent, followed by a 10% bleach sol

Luminex 100 IS User Manual Version 2.1 xMAP Technology1 - 2 PN 89-00002-00-070 Rev. ACustomer Support Luminex Customer Support representatives are rea

100 IS User Manual Version 2.1 xMAP Technology8 - 4 PN 89-00002-00-070 Rev. AFigure 100. Grasping the Tubing4. Remove the filter with one hand, and h

xMAP Technology Maintenance and CleaningPN 89-00002-00-070 Rev. A 8 - 5 Figure 101. Removing the Screen3. Replace the filter and replace the screen.F

100 IS User Manual Version 2.1 xMAP Technology8 - 6 PN 89-00002-00-070 Rev. ASyringe Seal To replace the syringe plunger seal:1. Open the center-most

xMAP Technology Maintenance and CleaningPN 89-00002-00-070 Rev. A 8 - 710. Hand-tighten the screw on the syringe arm.11. Plug in the power cord and tu

100 IS User Manual Version 2.1 xMAP Technology8 - 8 PN 89-00002-00-070 Rev. AAnnuallySheath Filter  To change the sheath filter within the analyzer:

xMAP Technology Maintenance and CleaningPN 89-00002-00-070 Rev. A 8 - 9As RequiredSheath and Waste Fluids Replace the sheath fluid and empty the waste

100 IS User Manual Version 2.1 xMAP Technology8 - 10 PN 89-00002-00-070 Rev. AIf the sheath container ever runs dry, prime the system at least twice u

xMAP Technology Maintenance and CleaningPN 89-00002-00-070 Rev. A 8 - 11Sample Arm Vertical HeightThe vertical height determines how far the XYP instr

100 IS User Manual Version 2.1 xMAP Technology8 - 12 PN 89-00002-00-070 Rev. A

Luminex 100 IS Maintenance Log Month(s): ________________________________________Year:_______________Maintenance LogProcedu

PN 89-00002-00-070 Rev. A 2 - 1SafetySymbols These symbols describe warnings, cautions, and general information used in the operation of this instrume

PN 89-00002-00-070 Rev. A 9 - 1 TroubleshootingTroubleshooting the Luminex 100 ISCustomer support is available to users in the U.S. and Canada by call

Luminex 100 IS User Manual Version 2.1 xMAP Technology9 - 2 PN 89-00002-00-070 Rev. AVerify the SystemWith SystemControls To verify your system:1. Vo

xMAP Technology TroubleshootingPN 89-00002-00-070 Rev. A 9 - 3Luminex 100 Analyzer Fails CalibrationPerform the following items in the order they appe

Luminex 100 IS User Manual Version 2.1 xMAP Technology9 - 4 PN 89-00002-00-070 Rev. AFailure During Self-Diagnostics TestsIf the self diagnostics rout

xMAP Technology TroubleshootingPN 89-00002-00-070 Rev. A 9 - 5System Error MessagesThe following error messages can be displayed in the message log on

Luminex 100 IS User Manual Version 2.1 xMAP Technology9 - 6 PN 89-00002-00-070 Rev. ALow Laser Power Detected• Warm up the system. This takes approxim

xMAP Technology TroubleshootingPN 89-00002-00-070 Rev. A 9 - 7Insufficient Bead Count • Do not select Cancel during sample acquisition. Selecting the

Luminex 100 IS User Manual Version 2.1 xMAP Technology9 - 8 PN 89-00002-00-070 Rev. A• Ensure that the correct control lot number and target values we

xMAP Technology TroubleshootingPN 89-00002-00-070 Rev. A 9 - 9Sample Empty Detected This error message occurs when the system completely acquires the

Luminex 100 IS User Manual Version 2.1 xMAP Technology2 - 2 PN 89-00002-00-070 Rev. ASafety Precautions Read the following safety information before s

Luminex 100 IS User Manual Version 2.1 xMAP Technology9 - 10 PN 89-00002-00-070 Rev. A• Complete the following analyzer functions from the Maintenance

xMAP Technology TroubleshootingPN 89-00002-00-070 Rev. A 9 - 11Heater Block Will Not Reach Desired Temperature• Ensure that the XYP instrument door is

Luminex 100 IS User Manual Version 2.1 xMAP Technology9 - 12 PN 89-00002-00-070 Rev. A

PN 89-00002-00-070 Rev. A 10 - 1Product NumbersHardware10Air Filter, Base, Hepa CN-0001-01Air Filter (on bottom of instrument)CN-0002-01Air Filter, I

Luminex 100 IS User Manual Version 2.1 xMAP Technology10 - 2 PN 89-00002-00-070 Rev. AMonitor CN-PC02-01Power Cord, USAPower Cord, AustraliaPower Cord

xMAP Technology Product NumbersPN 89-00002-00-070 Rev. A 10 - 3SoftwarexMAP ReagentsTrainingLuminex 100 IS software CD CN-SW05-01Microspheres, LX100,

Luminex 100 IS User Manual Version 2.1 xMAP Technology10 - 4 PN 89-00002-00-070 Rev. A

PN 89-00002-00-070 Rev. A 11 - 1Glossaryambient temperature The temperature of the surrounding environment.analyte A substance that is detected throug

Luminex 100 IS User Manual Version 2.1 xMAP Technology11 - 2 PN 89-00002-00-070 Rev. ACL2 Refers to dyes embedded in the microsphere. Also see classif

xMAP Technology GlossaryPN 89-00002-00-070 Rev. A 11 - 3laser Light Amplification by Stimulated Emission of Radiation (laser). This highly purified so

xMAP Technology SafetyPN 89-00002-00-070 Rev. A 2 - 3This label appears on the back of the XYP instrument.Figure 3. Fuse Replacement Warning Label -

Luminex 100 IS User Manual Version 2.1 xMAP Technology11 - 4 PN 89-00002-00-070 Rev. Asample reaction The reaction that occurs between your reagents a

PN 89-00002-00-070 Rev. A A - 1Luminex Sheath Delivery SystemIntended Use The Luminex SD™ (sheath delivery) system is designed as an accessory for use

Luminex 100 IS User Manual Version 2.1 xMAP TechnologyA - 2 PN 89-00002-00-070 Rev. A• Waste line tubing (orange fitting)• Sheath fluid line (blue fit

xMAP TechnologyPN 89-00002-00-070 Rev. A A - 33. Calibrate your system according to the instruction in the “System Calibration” chapter.4. From the Ho

Luminex 100 IS User Manual Version 2.1 xMAP TechnologyA - 4 PN 89-00002-00-070 Rev. A8. Lower the stainless steel filter end of the sheath fluid line

xMAP TechnologyPN 89-00002-00-070 Rev. A A - 512. Calibrate your system again and run the system controls. Check the calibration trend report. Compare

Luminex 100 IS User Manual Version 2.1 xMAP TechnologyA - 6 PN 89-00002-00-070 Rev. ARegularly, as required. Replace the sheath fluid as required.Week

xMAP TechnologyPN 89-00002-00-070 Rev. A A - 7This reverses the sheath flow and allows the reservoir to drain slowly and completely.SD system device i

Luminex 100 IS User Manual Version 2.1 xMAP TechnologyA - 8 PN 89-00002-00-070 Rev. A

PN 89-00002-00-070 Rev. A 1Symbols 4Aabout device 67about IS 68software 68access doors 7accessories 11see accessory instructions 11see user manual 11a

Luminex 100 IS User Manual Version 2.1 xMAP Technology2 - 4 PN 89-00002-00-070 Rev. AFigure 6. Luminex 100 Voltage Label (Uniphase) The Luminex 100 a

2 PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP Technologybackflush 21sheath fluid 21time 21background information 1analytes 1f

PN 89-00002-00-070 Rev. A 3xMAP Technologyconnect 3barcode reader 4keyboard 4mouse 4PC 4connect Luminex 100 instrument 1connect the XYP instrument 1co

4 PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP Technologyerrors tabdata interpretation window 5European Union (EU) safety requ

PN 89-00002-00-070 Rev. A 5xMAP Technologyproblem detected 12initialize the PC 12inquiriescustomer support 2via email 2email address 1install the XYP

6 PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP Technologymechanical warnings 7Menu barFile, Tools, Help 4message log 2, 3messa

PN 89-00002-00-070 Rev. A 7xMAP Technologyproblem diagnosissystem calibrators 1tools 1procedure 12process multiple plates 46process multiple sessions

8 PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP Technologyneedle adjust 10Sample Probe down button 30sample timed out 8sample t

PN 89-00002-00-070 Rev. A 9xMAP Technologyspecifications, vortex 7specified volume 53speedspecifications 3stability 2start a platebegins acquisition 5

10 PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP TechnologyUPScable 7UPS cable 7uptake 1user manual, conventions 1user-accessib

xMAP Technology SafetyPN 89-00002-00-070 Rev. A 2 - 5Luminex 100 IS Laser ClassificationThe Luminex 100 IS system classifies per FDA 21 CFR 1040.10 as

© LUMINEX CORPORATION, 2001-2003. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any

Luminex 100 IS User Manual Version 2.1 xMAP Technology2 - 6 PN 89-00002-00-070 Rev. AAll laser apertures are located within the Luminex 100 analyzer a

xMAP Technology SafetyPN 89-00002-00-070 Rev. A 2 - 7Bar Code Reader Laser This label appears on the bar code reader.Figure 12. Bar Code Reader Laser

Luminex 100 IS User Manual Version 2.1 xMAP Technology2 - 8 PN 89-00002-00-070 Rev. ABiologicalHeatBlue Indicator LightThe blue light above the Sample

xMAP Technology SafetyPN 89-00002-00-070 Rev. A 2 - 9Decontamination of the Luminex 100 IS for Return ShipmentSanitize and deconaminate the accessibl

xMAP Technology SafetyPN 89-00002-00-070 Rev. A 2 - 10

PN 89-00002-00-070 Rev. A 3 - 1Basic ConceptsBackground InformationxMAP technology is a versatile system that measures soluble analytes. The 100 IS pe

Luminex 100 IS User Manual Version 2.1 xMAP Technology3 - 2 PN 89-00002-00-070 Rev. APhotodiodes and a photomultiplier tube receive fluorescent signal

xMAP Technology Basic ConceptsPN 89-00002-00-070 Rev. A 3 - 3System calibrators and controls are provided as part of the system. Calibrate at least on

Luminex 100 IS User Manual Version 2.1 xMAP Technology3 - 4 PN 89-00002-00-070 Rev. A

PN 89-00002-00-070 Rev. A 4 - 1InstallationAssemble and Power the Luminex 100 IS SystemBefore installing the system, ensure that the facility complies

End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in

Luminex 100 IS User Manual Version 2.1 xMAP Technology4 - 2 PN 89-00002-00-070 Rev. Ainstrument. Do not plug the XYP instrument into the power outlet.

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 3Refer to the documentation that came with your PC to determine if changing the voltage sele

Luminex 100 IS User Manual Version 2.1 xMAP Technology4 - 4 PN 89-00002-00-070 Rev. AFigure 16. Luminex 100 IS Connections12. Connect the barcode rea

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 5Figure 17. Connecting the PC, Mouse, Barcode Reader, and Keyboard 13. Fill the sheath bott

Luminex 100 IS User Manual Version 2.1 xMAP Technology4 - 6 PN 89-00002-00-070 Rev. AInstall or Replace the XYP Instrument Sample Probe To install th

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 73. Remove the Cheminert fitting by turning the connector counterclockwise. If the tubing is

Luminex 100 IS User Manual Version 2.1 xMAP Technology4 - 8 PN 89-00002-00-070 Rev. AFigure 20. The XYP Instrument Sampling Probe6. Re-install the li

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 98. Plug in the XYP instrument and the Luminex 100 analyzer.Install the XYPInstrumentReservo

Luminex 100 IS User Manual Version 2.1 xMAP Technology4 - 10 PN 89-00002-00-070 Rev. Aheater block in the bracket inside the left access door of the L

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 11 To adjust the vertical height of the Sample Probe:1. Turn on the XYP instrument, the Lum

5. EXPORT RESTRICTIONS. You agree that you will not export or re-export the SOFTWARE PRODUCT to any country, person, entity, or end-user subject to U.

Luminex 100 IS User Manual Version 2.1 xMAP Technology4 - 12 PN 89-00002-00-070 Rev. AInitializationInitialize the PC Upon powering your system, the P

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 13IIsometric View Right Side ViewLuminex SD19.55 lb. (8.89 kg.)Luminex XYP32.05 lb. (10.48 k

xMAP Technology InstallationPN 89-00002-00-070 Rev. A 4 - 14

PN 89-00002-00-070 Rev. A 5 - 1The SystemTheory of Operation Luminex 100 IS technology is based on flow cell fluorometry with Luminex-developed innova

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 2 PN 89-00002-00-070 Rev. Aphoto diode (APD) detectors measure the excitation emission inten

xMAP Technology The SystemPN 89-00002-00-070 Rev. A 5 - 3Required Laboratory Reagents• Household bleach• 70% isopropanol• Mild detergentSoftware Lumin

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 4 PN 89-00002-00-070 Rev. AAccuracy and Precision • Sample uptake volume ± 5%• Classificatio

xMAP Technology The SystemPN 89-00002-00-070 Rev. A 5 - 5Luminex 100 Analyzer General • Operating temperature: 15°C to 30°C (59°F to 86°F)• Storage te

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 6 PN 89-00002-00-070 Rev. AElectronics • Reporter channel detection: A/D resolution 14 bits•

xMAP Technology The SystemPN 89-00002-00-070 Rev. A 5 - 7• Power cords: Power cords specific to the country of use are included in the system as requi

PN 89-00002-00-070 Rev. A iContentsIntroduction 1-1About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1Inte

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 8 PN 89-00002-00-070 Rev. A Figure 25. Luminex 100 IS SetupElectronicsPower InputModuleThe

xMAP Technology The SystemPN 89-00002-00-070 Rev. A 5 - 91. Air intake filter access door2. Power input module3. Power switch4. XYP instrument ventila

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 10 PN 89-00002-00-070 Rev. AXYP InstrumentSample ProbeA stainless steel Sample Probe acquire

xMAP Technology The SystemPN 89-00002-00-070 Rev. A 5 - 11Access Doors Two access doors are on the face of the Luminex 100 analyzer. The center door g

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 12 PN 89-00002-00-070 Rev. ASyringe Located in this compartment behind the center door, the

xMAP Technology The SystemPN 89-00002-00-070 Rev. A 5 - 13Air, Waste, andSheath FluidConnectorsLocated on the side of the instrument, these connectors

Luminex 100 IS User Manual Version 2.1 xMAP Technology5 - 14 PN 89-00002-00-070 Rev. AOptical The optical system consists of the optical assembly and

PN 89-00002-00-070 Rev. A 6 - 1System CalibrationSystem Calibration and Verification OverviewxMAP calibrator microspheres are used to normalize the se

Luminex 100 IS User Manual Version 2.1 xMAP Technology6 - 2 PN 89-00002-00-070 Rev. ACalibrate the SystemRun SystemCalibrators To calibrate your syst

xMAP Technology System CalibrationPN 89-00002-00-070 Rev. A 6 - 3Figure 33. Update Calibration Targets Dialog Box10. Enter the values listed from the

ii PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP TechnologyThe System 5-1Theory of Operation . . . . . . . . . . . . . . . . .

Luminex 100 IS User Manual Version 2.1 xMAP Technology6 - 4 PN 89-00002-00-070 Rev. ARun SystemControlsTo run system controls, follow these steps:1. C

xMAP Technology System CalibrationPN 89-00002-00-070 Rev. A 6 - 56. Click OK and wait until CON1 completes. The device status section in the status ba

Luminex 100 IS User Manual Version 2.1 xMAP Technology6 - 6 PN 89-00002-00-070 Rev. APrint or View aCalibration orSystem ControlTrend ReportYou must e

xMAP Technology System CalibrationPN 89-00002-00-070 Rev. A 6 - 73. Select the product for the trend report and click the Next button. A new dialog bo

Luminex 100 IS User Manual Version 2.1 xMAP Technology6 - 8 PN 89-00002-00-070 Rev. A

PN 89-00002-00-070 Rev. A 7 - 1Software TourLuminex 100 IS 2.1 SoftwareThe Luminex 100 IS (IS 2.1) software starts automatically when you turn on the

100 IS S Users Manual Version 2.1 xMAP Technology7 - 2 PN 89-00002-00-070 Rev. AThe calibration and control target values can be found on the certific

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 3IS 2.1 Main Screen After entering the calibration and control target values, the main scre

100 IS S Users Manual Version 2.1 xMAP Technology7 - 4 PN 89-00002-00-070 Rev. AMenu Bar The main screen in the IS 2.1 software has three menus: File,

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 54. Enter a new batch directory if you want the batch located in a location other than C:\M

PN 89-00002-00-070 Rev. A iiixMAP TechnologySystem Calibration 6-1System Calibration and Verification Overview . . . . . . . . . . . . . . . 6-1Calib

100 IS S Users Manual Version 2.1 xMAP Technology7 - 6 PN 89-00002-00-070 Rev. AToolbar The main screen has a toolbar that contains several buttons to

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 7The following table describes the color coding with relation to types of message received

100 IS S Users Manual Version 2.1 xMAP Technology7 - 8 PN 89-00002-00-070 Rev. ABoth template and manual commands appear on the status bar as the syst

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 9Tabs The main screen contains four tabs: Home, Run Batch, Maintenance, and Diagnostics. Th

100 IS S Users Manual Version 2.1 xMAP Technology7 - 10 PN 89-00002-00-070 Rev. AHome Tab The home tab appears as part of the main screen and contains

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 11The templates and commands appear in alphabetical order for easy locating.Figure 48. Fav

100 IS S Users Manual Version 2.1 xMAP Technology7 - 12 PN 89-00002-00-070 Rev. AAdd Commands toFavorites To add commands to the favorites section:1.

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 13Remove ItemsFrom Favorites To remove items from the favorites section:1. Select the item

100 IS S Users Manual Version 2.1 xMAP Technology7 - 14 PN 89-00002-00-070 Rev. ARun Batch Tab The Run Batch tab contains an information area for batc

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 15Reservoir The reservoir holds fluids used in system maintenance operations such as washin

iv PN 89-00002-00-070 Rev. ALuminex 100 IS User Manual Version 2.1 xMAP TechnologyCalibration and Verification . . . . . . . . . . . . . . . . . . .

100 IS S Users Manual Version 2.1 xMAP Technology7 - 16 PN 89-00002-00-070 Rev. ASet the XYPInstrument HeaterTemperatureRefer to your assay kit instru

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 17command list also tells you whether the command completed successfully or if it failed.Fi

100 IS S Users Manual Version 2.1 xMAP Technology7 - 18 PN 89-00002-00-070 Rev. AMaintenance Tab The maintenance tab lets you perform several maintena

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 19Table 3. Maintenance Operations: Recommended Use ScheduleMaintenance OperationRecommende

100 IS S Users Manual Version 2.1 xMAP Technology7 - 20 PN 89-00002-00-070 Rev. AIf an error occurs, the Diagnostics tab appears (with the tab heading

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 21Prime the System Prime the system as necessary to remove air from the system’s fluidic pa

100 IS S Users Manual Version 2.1 xMAP Technology7 - 22 PN 89-00002-00-070 Rev. AAlcohol Flush theSystemAlcohol flush the system to remove air bubbles

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 23Wash the System Use the wash cycle as needed using sheath fluid. For example, wash with s

100 IS S Users Manual Version 2.1 xMAP Technology7 - 24 PN 89-00002-00-070 Rev. ADrain the System The cuvette in the system can be drained temporarily

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 25 To soak the probe:1. Click Soak. A confirmation dialog box appears.Figure 58. Confirma

PN 89-00002-00-070 Rev. A vxMAP TechnologyReports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-60Export Data

100 IS S Users Manual Version 2.1 xMAP Technology7 - 26 PN 89-00002-00-070 Rev. ARun SelfDiagnosticsRun self diagnostics to see if the device and all

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 27View Failed Self-Diagnostic Details To view details regarding which of the self-diagnost

100 IS S Users Manual Version 2.1 xMAP Technology7 - 28 PN 89-00002-00-070 Rev. A1. Click New CAL Targ. from the Maintenance tab. An Update Cal Target

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 29Enter SystemControl Targets To enter new and changed control targets:1. Click New CON Ta

100 IS S Users Manual Version 2.1 xMAP Technology7 - 30 PN 89-00002-00-070 Rev. AEject and Retractthe XYPInstrument PlateHolder To eject the XYP inst

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 31These tools help you find information about the system and what happens during sample acq

100 IS S Users Manual Version 2.1 xMAP Technology7 - 32 PN 89-00002-00-070 Rev. AThe system monitor provides values and units of measure for each prop

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 33and commands that the system has performed as well as each operation’s progress, time and

100 IS S Users Manual Version 2.1 xMAP Technology7 - 34 PN 89-00002-00-070 Rev. Aclick that row. An error dialog box appears with details about that e

xMAP Technology Software TourPN 89-00002-00-070 Rev. A 7 - 35Figure 66. Import Template Dialog Box3. Select your floppy disk drive as the Look in loc