Luminex xMAP Antibody Coupling Kit Manuale Utente

xMAP® AntibodyCoupling KitUser ManualProduct Catalog NumberxMAP® Antibody Coupling Kit40-50016For Research Use OnlyNot For Use In Diagnostic Procedure

Page 6 of 20Storage and StabilityPhotosensitive microspheres should be protected from light at all times. All kit components are to be stored at 2-8ºC

Page 7 of 20• Vortexer• Pipettor Optional Equipment• xMAP Antibody Coupling Kit Quick Guide (89-30000-00-361) (Available at http://www.luminexcorp.com

Page 8 of 20InstructionsGeneral WorkflowCoupling ProtocolThe following is the detailed step-by-step process for coupling antibodies (or similar protei

Page 9 of 204. Dispense the desired amount of microspheres from the stock vial into one of the microcentrifuge tubes (“reaction tube”) provided with t

Page 10 of 207. Place the reaction tube with microspheres into the magnetic separator for 1-2 minutes and, with the tube still positioned in the magne

Page 11 of 2016. Wash the microspheres.A. Place the reaction tube with microspheres into the magnetic separator for 1-2 minutes.B. With the tube still

Page 12 of 2020. Calculate the volume of Activation Buffer needed for the reaction.• If coupling more than 5x106 microspheres, subtract the volume of

Page 13 of 20 NOTE: For optimal performance allow the coupled microspheres to block over-night before first use.

Page 14 of 20Coupling AssessmentOnce the coupling reaction has been completed, the coupled microspheres can be enumerated and the efficiency of the co

Page 15 of 204. Prepare a solution of phycoerythrin-labeled anti-species IgG detection antibody at 4 μg/mL in PBS-1% BSA. Prepare a 1:2 dilution serie

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Page 16 of 208. Mix the reactions gently by pipetting up and down several times with a pipettor.9. Cover the plate and incubate for 30 minutes at room

Page 17 of 20TroubleshootingThis troubleshooting guide may be helpful in solving problems that may arise. For more information, call Technical Support

Page 18 of 20Low/No signalsThe reagents may not have been stored correctlyStore all reagents as recommended.EDC may not have been stored correctly or

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Safety... 1Introduction ...

Page 1 of 20SafetyThe following informational notes and cautions are used in this manual. NOTE: This message is used to provide general helpful inform

Page 2 of 20Material Safety Data Sheets (MSDS's) are available upon request. Visit http://www.luminexcorp.com/support for more information.CAUTIO

Page 3 of 20IntroductionThe xMAP Antibody Coupling (AbC) Kit contains all of the reagents necessary to covalently couple antibodies to Luminex MagPlex

Page 4 of 20With Luminex MagPlex microspheres, if the kit protocol is performed carefully, the percent recovery of the coupling reaction is typically

Page 5 of 20Protein ConsiderationsThis kit includes a specially formulated Activation Buffer (pH 6.0) which is suitable for most antibodies. The prote